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Place a drop of the yeast culture onto a microscope slide using a pipet. Ad a drop of crystal violet stain to the rop of yeast culture on the microscope slide. [Then a]dd a cover slip over the yeast culture/stain fluid.
- Place the slide with smear(s)s up on a rack in a staining tray. You
may find it convenient to leave the clothes pin attached. Flood the
slide with Gram's crystal violet so that all of the dried material is
covered. It is good practice to flood the entire slide from edge to
- After 60 sec pick up the slide using the clothes pin and
rinse the smear with water by squirting the slide above the smear and
letting the water wash
over it until the water runs clear. To keep the slide "clean"
you might rinse the bottom as well, and make sure you have not trapped
stain underneath the clothes pin where you have grabbed the slide. Note
that the smears will be stained a bright blue.